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BTU
A practical approach to hedgerow management for improving the provision of ecosystem services
(2019)
Agroforestry is receiving increasing attention in Germany because of its capacity to address some of the negative consequences of intensive agriculture. However the actual uptake and maintenance of agroforestry by farmers remains low. The aim of this study was to identify the opportunities and obstacles to agroforestry in Germany and to identify potential measures which could address the obstacles and increase agroforestry adoption. Qualitative interviews with 32 farmers indicated that the legal framework and administrative burden associated with agroforestry in Germany was a major obstacle. The farmers recognised that agroforestry provided environmental benefits, but these do not always increase farm profitability. Agroforestry was identified as a viable alternative to conventional farming systems, especially in less productive areas, not profitable when managed in a conventional way. To enhance the implementation of agroforestry the simplification of the legal framework is crucial. Modern agroforestry should be better recognized by existing policy measures providing payments for environmentally friendly farming. Ultimately, an integrated approach for environmental benefits and production objectives is required, which is based on rewarding farmers for providing ecosystem services (ESs) and a stronger public involvement in sustainable land use practices. Rewarding farmers for ESs could increase the interest in agroforestry in highly productive areas, enhancing sustainable land use in the long term.
Intestinal colonization is influenced by the ability of the bacterium to inhabit a niche, which is based on the expression of colonization factors. Escherichia coli carries a broad range of virulence-associated genes (VAGs) which contribute to intestinal (inVAGs) and extraintestinal (exVAGs) infection. Moreover, initial evidence indicates that inVAGs and exVAGs support intestinal colonization. We developed new screening tools to genotypically and phenotypically characterize E. coli isolates originating in humans, domestic pigs, and 17 wild mammal and avian species. We analyzed 317 isolates for the occurrence of 44 VAGs using a novel multiplex PCR microbead assay (MPMA) and for adhesion to four epithelial cell lines using a new adhesion assay. We correlated data for the definition of new adhesion genes. inVAGs were identified only sporadically, particularly in roe deer (Capreolus capreolus) and the European hedgehog ( Erinaceus europaeus). The prevalence of exVAGs depended on isolation from a specific host. Human uropathogenic E. coli isolates carried exVAGs with the highest prevalence, followed by badger (Meles meles) and roe deer isolates. Adhesion was found to be very diverse. Adhesion was specific to cells, host, and tissue, though it was also unspecific. Occurrence of the following VAGs was associated with a higher rate of adhesion to one or more cell lines: afa-dra, daaD, tsh, vat, ibeA, fyuA, mat, sfa-foc, malX, pic, irp2, and papC. In summary, we established new screening methods which enabled us to characterize large numbers of E. coli isolates. We defined reservoirs for potential pathogenic E. coli. We also identified a very broad range of colonization strategies and defined potential new adhesion genes.
Abstract
BACKGROUND:
Different strategies of colonization or infection by E. coli result in formation of certain adhesion patterns which help also in classifying intestinal E. coli into pathotypes. Little is known about adhesion patterns and host- and tissue adaption of commensal E. coli and about E. coli originating in clinically healthy hosts carrying pathotype-specific virulence-associated genes.
FINDINGS:
Adhesion pattern of E. coli (n = 282) from humans and from 18 animal species were verified on intestinal human Caco-2 and porcine IPEC-J2 cells and, furthermore, for comparison on human urinary bladder 5637, porcine kidney PK-15 epithelial and HEp-2 cells. The analysis was carried out on 150,000 images of adhesion assays.Adhesion patterns were very diverse; 88 isolates were completely non-adherent, whereas 194 adhered to at least one cell line with the dominant adhesion patterns "diffusely distributed" and "microcolony formation". Adhesion patterns "chains" and "clumps" were also visible. Chain formation was mediated by the presence of epithelial cells. Clump formation was very specific on only the 5637 cell line. All enteropathogenic (eae+) E. coli (EPEC; n = 14) were able to form microcolonies which was cell line specific for each isolate. Most EPEC formed microcolonies on intestinal IPEC-J2 and Caco-2 but several also on urinary tract cells. Shigatoxin-producing (stx+) E. coli (n = 10) showed no specific adhesion patterns.
CONCLUSIONS:
E. coli isolates were highly diverse. Commensal and pathogenic isolates can adhere in various forms, including diffuse distribution, microcolonies, chains and clumps. Microcolony formation seems to be a global adhesion strategy also for commensal E. coli.
Alley cropping system is an agroforestry practice in which arable crops are cultivated between tree hedgerows. For more than 15 ye ars we have been doing research in agroforestry which has been carried out on different experimental sites in the field covering about 250 acres of alley cropping, more areas with short rotation coppice, and other tree-based field experiments located close to C ottbus / Germany. The presence of woody species in the alley cropping production system has been shown to contribute significantly to carbon sequestration, nutrient cycling, improved water availability for crops, stimulation of higher soil faunal activities, soil erosion control, soil fertility improvement and sustained levels of crop production.
Alley cropping systems have also come into focus for reclamation of post-mining areas where the initial content of soil organic matter (SOM) is generally close to zero and soil fertility is very low. The increase of SOM in reclamation areas depends on amount of biomass production and return to soil, and mechanisms of C protection. Production of Robinia pseudoacacia L. under alley cropping system has received considerable interest in these areas as an alternative to agricultural crops and an additional wood source, while acting as a potential C sink to counterbalance greenhouse gases emissions. The woody biomass accumulation of R. pseudoacacia reached 29.8 t drymass ha-1 after 6 years of establishment on reclaimed mine sites in northeastern Germany. Due to its high potential of litterfall production and N2 fixation, R. pseudoacacia contributed to improve soil physical, chemical and biological properties though increasing SOM, converting spoils into productive and sustainable soils. For this reason, in the temperate zone, agroforestry systems attract more and more public attention as they offer a promising and comprising way for adapting agricultural production to climate change and providing comprehensive ecosystem services. A comprehensive assessment of the ecosystem services in agroforestry systems in temperate regions should consider the potential to produce biomass and food, the evaluation of the carbon and nutrient budgets, the assessment of the potential impact of agroforestry on biodiversity at landscape scale and finally the exploration of the sustainability functions and socio-economic cross-cutting issues. These key parameters are relevant to policy by providing current and reliable information on the interactions between land management, ecosystem services and society.