Study of single and dual NIR fluorescently labeled nucleic acid conjugates for nucleic acid detection

  • In this research, a near-IR fluorescent labeled nucleic acid conjugate for the nucleic acid detection was synthesized, and characterized preliminarily for the detection of the nucleic acid. Th conjugate combines the molecular recognition properties of the oligonucleotides with the near-IR fluorescence label PR646. Both single- and dual- labeled conjugates were studied for their hybridization with the complementary nucleic acid. The dual labeled conjugate has indicated that the self-quenching effect exists in ssDNA form while the fluorescence increases greatly after hybridization with the complementary nucleic acids. The time-resolved fluorescence was also studied.

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Author: Zhihong Lin, Meng Wu, Shu Ren, Michaela Arbter, Martin Boehmer, Vladimir M. MirskyORCiDGND, Otto S. WolfbeisGND
DOI:https://doi.org/10.1117/12.440165
ISBN:0-8194-4119-8
Title of the source (English):Proceedings, International Conference on Sensor Technology (ISTC 2001), 10 - 12 October 2001, Wuhan, China
Publisher:SPIE
Place of publication:Bellingham, Wash.
Editor: Yikai Zhou
Document Type:Conference Proceeding
Language:English
Year of publication:2001
Tag:Near infrared; luminescence
First Page:111
Last Page:114
Series ; volume number:SPIE Proceedings ; 4414
Faculty/Chair:Fakultät 2 Umwelt und Naturwissenschaften / FG Nanobiotechnologie
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