Electrostatic assay of phospholipase A activity: an application of the second harmonic method of monitoring membrane boundary potentials
- To evaluate phospholipase A activity a new assay is suggested. This assay is based on the recording of boundary potential changes of the planar bilayer lipid membrane during enzymatic hydrolysis of lipids. To register these changes, a second harmonic method is used. Sensitivity of the assay is about 0.0002 units/ml regardless of the impurities that may be present in the samples. One analysis takes about 5 min.
Author: | Vladimir M. MirskyORCiDGND, Vladimir V. Cherny, Valeri S. Sokolov, Vladislav S. Markin |
---|---|
ISSN: | 0165-022X |
ISSN: | 1872-857X |
Title of the source (English): | Journal of Biochemical and Biophysical Methods |
Document Type: | Scientific journal article peer-reviewed |
Language: | English |
Year of publication: | 1990 |
Volume/Year: | 21 |
Issue number: | 4 |
First Page: | 277 |
Last Page: | 284 |
Faculty/Chair: | Fakultät 2 Umwelt und Naturwissenschaften / FG Nanobiotechnologie |