Electrostatic assay of phospholipase A activity: an application of the second harmonic method of monitoring membrane boundary potentials

  • To evaluate phospholipase A activity a new assay is suggested. This assay is based on the recording of boundary potential changes of the planar bilayer lipid membrane during enzymatic hydrolysis of lipids. To register these changes, a second harmonic method is used. Sensitivity of the assay is about 0.0002 units/ml regardless of the impurities that may be present in the samples. One analysis takes about 5 min.

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Metadaten
Author: Vladimir M. MirskyORCiDGND, Vladimir V. Cherny, Valeri S. Sokolov, Vladislav S. Markin
ISSN:0165-022X
ISSN:1872-857X
Title of the source (English):Journal of Biochemical and Biophysical Methods
Document Type:Scientific journal article peer-reviewed
Language:English
Year of publication:1990
Volume/Year:21
Issue number:4
First Page:277
Last Page:284
Faculty/Chair:Fakultät 2 Umwelt und Naturwissenschaften / FG Nanobiotechnologie
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