Capacitive sensor for lipolytic enzymes

  • The preparation of the capacitive biosensor for monitoring the activity of enzymes (lipases, phospholipases, etc.) hydrolysing waterinsoluble substrates into water-soluble products is described. The sensor is based on a sandwich-like structure: Au/S(CH₂)₁₇CH₁/substrate/electrolyte. Hydrolysis of the substrate leads to the formation of water-soluble products and desorption of these compounds from the electrode. When the product formation is the rate-limiting step of this process, the desorption rate is determined by the enzyme activity. The desorption can be easily monitored as an increase of the electrode capacitance. By following this principle it was possible to develop a sensor monitoring phospholipase A₂ activity even at the 50 pg ml-1 level.

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Metadaten
Author: Vladimir M. MirskyORCiDGND, Christian Krause, Klaus Dietrich Heckmann
DOI:https://doi.org/10.1016/S0040-6090(95)08485-1
ISSN:0040-6090
Title of the source (English):Thin Solid Films
Document Type:Scientific journal article peer-reviewed
Language:English
Year of publication:1996
Tag:Capacitive sensor; Enzyme assay; Phospholipase AZ; Self-assembly layer
Volume/Year:284-285
First Page:939
Last Page:941
Faculty/Chair:Fakultät 2 Umwelt und Naturwissenschaften / FG Nanobiotechnologie
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