Covalent immobilization of oligonucleotides on electrodes

  • 32P-labeled DNA oligomers were immobilized onto self-assembled monolayers formed by carboxy-modified alkylthiols on gold electrodes. The optimal conditions were evaluated by comparing of surface density of oligonucleotides measured for different immobilization conditions before and after desorption of physically adsorbed nucleotides. The results demonstrate that the physical adsorption depends strongly on immobilization conditions being typically 10 times higher than the chemical immobilization. Under optimal immobilization conditions, the surface density of oligonucleotides was more than 20% of the value calculated for a densely packed monolayer.

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Author: Nadia Wrobel, Werner Deininger, Peter Hegemann, Vladimir M. MirskyORCiDGND
DOI:https://doi.org/10.1016/S0927-7765(03)00155-3
ISSN:0927-7765
Title of the source (English):Colloids and Surfaces B: Biointerfaces
Document Type:Scientific journal article peer-reviewed
Language:English
Year of publication:2003
Tag:Biosensor; DNA; DNA immobilization; DNA sensor; Hybridization
Volume/Year:32
Issue number:2
First Page:157
Last Page:162
Faculty/Chair:Fakultät 2 Umwelt und Naturwissenschaften / FG Nanobiotechnologie
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