Anomalous adsorptive properties of HIV protease: Indication of two-dimensional crystallization?
- Adsorption of HIV protease onto surfaces that are usually considered to be protein-resistant was studied quantitatively using surface plasmon resonance. Adsorption onto gold surfaces functionalized by OH-terminated alkyl chains was much stronger than onto oligo(ethylene glycol)-terminated surfaces. Equilibrium and kinetic adsorption constants were determined. An anomalous mutual attraction between adsorbate molecules was observed, indicating the possibility of two-dimensional crystallization of HIV protease. These results are applicable for the design of sensors/biosensors for HIV protease resistance detection and for proper manipulation of this enzyme in laboratory devices.
Author: | Petr Cígler, Vladimír Král, Milan Kožíšek, Jan Konvalinka, Vladimir M. MirskyORCiDGND |
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DOI: | https://doi.org/10.1016/j.colsurfb.2008.01.011 |
ISSN: | 0927-7765 |
Title of the source (English): | Colloids and Surfaces B: Biointerfaces |
Document Type: | Scientific journal article peer-reviewed |
Language: | English |
Year of publication: | 2008 |
Tag: | HIV protease; Protein adsorption; Protein-resistant surfaces; Self-assembled monolayer; Surface plasmon resonance (SPR) |
Volume/Year: | 64 |
Issue number: | 1 |
First Page: | 145 |
Last Page: | 149 |
Faculty/Chair: | Fakultät 2 Umwelt und Naturwissenschaften / FG Nanobiotechnologie |