TY - GEN A1 - Krümmelbein, Julia A1 - Bens, Oliver A1 - Raab, Thomas A1 - Naeth, M. Anne T1 - A history of lignite coal mining and reclamation practices in Lusatia, eastern Germany T2 - Canadian journal of soil science Y1 - 2012 SN - 1918-1841 VL - 92 IS - 1 SP - 53 EP - 66 ER - TY - GEN A1 - Raab, Thomas A1 - Krümmelbein, Julia A1 - Schneider, Anna A1 - Gerwin, Werner A1 - Maurer, Thomas A1 - Naeth, M. Anne T1 - Initial Ecosystem Processes as Key Factors of Landscape Development - A Review T2 - Physical Geography Y1 - 2012 SN - 1930-0557 VL - 33 IS - 4 SP - 305 EP - 343 ER - TY - GEN A1 - Distel, Henrik A1 - Hirsch, Florian A1 - Raab, Thomas A1 - Naeth, M. Anne T1 - Effects of slope aspect on soil development in the Canadian prairies: a digital approach T2 - European Geosciences Union, General Assembly 2019, Vienna, Austria, 7–12 April 2019 Y1 - 2019 UR - https://meetingorganizer.copernicus.org/EGU2019/EGU2019-3291.pdf CY - Katlenburg-Lindau ER - TY - CHAP A1 - Raab, Thomas A1 - Hirsch, Florian A1 - Vasserman, Oleksandr A1 - Raab, Alexandra A1 - Naeth, M. Anne T1 - Influence of relief and vegetation on soil properties in a disturbed chernozem soil landscape T2 - European Geosciences Union, General Assembly 2017, Vienna, Austria N2 - In central and southeastern Alberta, chernozems dominate the soil landscape and are divided into several groups that follow the climate gradient from Northwest to Southeast: Dark Grey Chernozems, Black Chernozems, Dark Brown Chernozems; Brown Chernozems. Principles controlling development and distribution of these chernozem subtypes along the ecotone transect are quite well known. However, intensive land use over the last century has affected soils that originally have formed under natural conditions during the Holocene in more than 10,000 years. There is a lack of knowledge regarding soil development in these landscapes on the decadal to centennial time scale. Within this time frame the most important factor of soil formation may be relief, although this has not been properly studied. This study aims to compare soil properties in a typical chernozem landscape where soils have been highly disturbed and parent materials have been re-arranged by surface coal mining.We hypothesize that within 50 years, soils develop with significant differences based on vegetation type and slope aspect. Our study site is the former Diplomat Mine near Forestburg, Alberta where spoils form a small scale ridge and graben topography. The south facing slopes of the piles are covered by grassland, whereas on the north exposition has trees and shrubs. Samples were taken from six sites with differences in topography and vegetation type. Diplomate T1 is at the top of the ridge with grassland, Diplomate S1 is on the southern slope with grassland, Diplomate N1 is on the northern slope with trees, and Diplomate N2 is on the northern slope with shrubs. For comparison we took samples from two sites without slope aspect. One site was an undisturbed grassland (Diplomate Z1) and the other sites were reclaimed piles (Diplomate R1). At each site, five soil profiles were examined and volumetrically sampled (250 cm3 steel ring) in steps of five centimeters to a depth of 30 centimeters. We present first results of basic physical and chemical soil parameters (bulk density, water content, pH, Cstock, N-stock). Y1 - 2017 UR - http://meetingorganizer.copernicus.org/EGU2017/EGU2017-2332.pdf N1 - EGU2017-2332 PB - European Geophysical Society CY - Katlenburg-Lindau ER - TY - CHAP A1 - Raab, Thomas A1 - Naeth, M. Anne A1 - Hirsch, Florian A1 - Raab, Alexandra T1 - Contributing towards a conceptual model of soil-landscape co-evolution: observations from historic mining sites in Alberta, Canada T2 - European Geosciences Union, General Assembly 2016, Vienna, Austria N2 - At the former Diplomat Mine near Forestburg, Alberta, Canada we find a diverse soil landscape which can help to conceptualize factors and processes controlling initial pedogenesis and soil distribution on very young landforms in prairie environments. Due to differing reclamation practices in the 1950s and landslides occurring after spoil dumping, four areas can be distinguished by GoogleMaps/LiDAR evaluation and onsite field survey: (i) not-mined, (ii) stock piled and unreclaimed, (iii) stock piled and reclaimed and (iv) affected by post-mining geomorphodynamics and quasi-natural redeposition. The parent material for areas (ii) to (iv) was initially dumped by spreaders but only (ii) didn’t undergo further change. Landscape (iii) has seen levelling of the piles by heavy machinery. Features of landscape (iv) are formed by reshaping the originally dumped and levelled structures. This last landscape unit marks the rim of the former mine adjacent to the river valley. In practice, mining activities formed new valley slopes. In contrast to the naturally developed slopes the mine slopes were less stable. Vegetation, which could have hindered slope wash erosion, was missing after dumping the spoil slopes. Slopes were very steep (or practically undercut) and therefore, substrates were naturally re-located by mass movements such as sliding and slumping. Characteristic sliding and slumping structures can be identified in the close-ups of the LiDAR images. Both processes, mass movement and slope wash erosion, may have overlapped. Landscape (ii) is the most contrasting one. Dumped stock piles formed elongated, curved and steep ridges. These landforms do not have a natural analogue but clearly show their technological origin. Most interesting are differences in vegetation. South and southwest facing slopes are covered with grassland whereas north and northeast facing slopes are covered with aspen trees. Some of the ditches are filled with water and form small elongated ponds. The characteristic geomorphology of the prairie can be found in landscape (i). Distinct differences are found in properties and types of soils in these four landscapes. Natural soils and pile soils characteristically differ in parent material and soil horizons. No information is gathered yet for the reclaimed soils and the landslides soils due to prohibited access. However, based on what we find at the former Diplomat Mine we can conclude that distribution, development and properties of unreclaimed soils in historical open cast mines in Alberta are primarily controlled by parent material and topography. The geomorphological set-up is dominating the trajectory of vegetation development and post-mining geomorphodynamics. Contrasting slope aspects determine micro climatic conditions and lead to different vegetation types. This has likely had an effect on soil development and soil properties (especially carbon stocks). Further studies will be conducted to quantify these differing soil properties to refine this conceptual model of initial pedogenesis and soil distribution on very young landforms in the prairie landscapes. Y1 - 2016 UR - http://meetingorganizer.copernicus.org/EGU2016/EGU2016-2457.pdf N1 - EGU2016-2457 PB - European Geophysical Society CY - Katlenburg-Lindau ER - TY - GEN A1 - Hirsch, Florian A1 - Raab, Thomas A1 - Distel, Henrik A1 - Naeth, M. Anne T1 - Dynamics of pedogenic carbonates and their relation to slope aspects in the Rumsey area (Alberta, Canada) T2 - European Geosciences Union, General Assembly 2019, Vienna, Austria, 7–12 April 2019 Y1 - 2019 UR - https://meetingorganizer.copernicus.org/EGU2019/EGU2019-2302-1.pdf CY - Katlenburg-Lindau ER - TY - GEN A1 - Hirsch, Florian A1 - Raab, Thomas A1 - Distel, Henrik A1 - Naeth, M. Anne A1 - Bonhage, Alexander T1 - Modelling Late Quaternary Soil Development Considering Slope Aspects in the Rumsey Area (Alberta, Canada) T2 - Soil Science Society of America, International Soils Meeting, Soils Across Latitudes, San Diego 2019 Y1 - 2019 UR - https://scisoc.confex.com/scisoc/2019sssa/meetingapp.cgi/Paper/114849 ER - TY - CHAP A1 - Boldt-Burisch, Katja A1 - Naeth, M. Anne T1 - The influence of arbuscular mycorrhizae on root precision nutrient foraging of two pioneer plant species during early reclamation T2 - European Geosciences Union General Assembly 2017 Vienna, Austria, 23–28 April 2017 N2 - On many post mining sites in the Lusatian Mining District (East Germany) soil heterogeneity consists of sandy soil with embedded clay-silt fragments. Those clays silt fragments might act as nutrient hotspots. Arbuscular mycorrhizal fungi in an infertile ecosystem could enhance a plant’s ability to selectively forage for those nutrients and thus to improve plants nutrient supply. In our study we investigated whether silt-clay fragments within a sandy soil matrix induced preferential root growth of Lotus corniculatus and Calamagrostis epigeios, whether arbuscular mycorrhizae influenced root foraging patterns, and to what extent selective rooting in clay silt fragments influenced plant growth were addressed in this research. Soil types were sterile and non-sterile sandy soil and clay-silt fragments. Treatments were with and without arbuscular mycorrhizae, with and without soil solution, and soil solution and mycorrhizal inoculum combined. Root biomass, root density and intraradical fungal alkaline phosphatase activity and frequency were determined in fragments relative to sandy soil. Furthermore, temporal relationship of number of roots in fragments and plant height was assessed. Lotus corniculatus showed strong selective rooting into fragments especially with those plants treated with commercial cultivated arbuscular mycorrhizae; Calamagrostis epigeios did not. Without arbuscular mycorrhizae, L. corniculatus growth was significantly reduced and selective rooting did not occur. Selective rooting induced significant growth spurts of L. corniculatus. Roots in fragments had higher fungal alkaline phosphatase activity suggesting that mycorrhizal efficiency and related plants phosphorus supply is enhanced in roots in fragments. The application of cultivated arbuscular mycorrhizal fungi significantly and quickly influenced root foraging patterns, especially those of L. corniculatus, suggesting mycorrhizae may also enhance the ability of other plants to selectively forage for nutrients and could therefore play an important role in early plant establishment on infertile reclamation sites. Y1 - 2017 UR - http://meetingorganizer.copernicus.org/EGU2017/EGU2017-12570.pdf N1 - EGU2017-12570 PB - European Geophysical Society CY - Katlenburg-Lindau ER - TY - GEN A1 - Boldt-Burisch, Katja A1 - Schneider, Bernd Uwe A1 - Naeth, M. Anne A1 - Hüttl, Reinhard F. T1 - Root exudation of organic acids of herbaceous pioneer plants and their growth in steriled and non-steriled nutrient-poor, sandy soils from post-mining sites T2 - Pedosphere N2 - Nutrient-poor, sandy soils form the prevailing substrate at post-mining sites and present a challenge for plants. We studied the organic acid quantity and composition of three commonly occurring pioneer plant species, the legumes Lotus corniculatus L. and Trifolium arvense L. and the grass Calamagrostis epigeios (L.) Roth, to determine if plant growth and exudation differed with (nonsterile soil) and without (sterile soil) an indigenous soil microbial community. We investigated whether organic acids were found in the rhizosphere and surrounding soil and whether this influenced nutrient mobilization. This study consists of linked field investigations and a greenhouse experiment. Plants were grown in the greenhouse in either steriled or non-steriled sandy soil from a reclamation site in the Lusatian mining landscape (Welzow S¨ud, East Germany). After seven months, the plant biomass, root morphology, organic acids, and water-soluble nutrients and root colonization with arbuscular mycorrhizal fungi (AMF) and dark septate endophytes (DSE) were analyzed. Roots of all three plants in the field and greenhouse experiments were highly colonized with AMF. Calamagrostis epigeios and T. arvense had a significantly higher colonization frequency with DSE than L. corniculatus. The quantity and composition of organic acids strongly differed among plant species, with the highest number of organic acids found for L. corniculatus and lowest for C. epigeios. The quantity of organic acids was greatly reduced in all plants under steriled soil conditions. However, the composition of organic acids and plant growth in steriled soil were reduced for both legumes, but not for C. epigeios, which had a higher biomass under steriled conditions. Changes in nutrient concentrations in the field rhizosphere soil relative to those in the control were measurable after seven months. While the spectrum of organic acids and the growth of legumes seemed to be dependent on a highly diverse soil microbial community and a symbiotic partner, the grass C. epigeios appeared capable of mobilizing enough nutrients without an indigenous microbial community, and might be more competitive on sites where soil microbial diversity and activity are low. Y1 - 2019 U6 - https://doi.org/10.1016/S1002-0160(18)60056-6 SN - 1002-0160 VL - 29 IS - 1 SP - 34 EP - 44 ER - TY - GEN A1 - Boldt-Burisch, Katja A1 - Naeth, M. Anne A1 - Schneider, Bernd Uwe A1 - Hüttl, Reinhard F. T1 - Linkage between root system development of three pioneer plant species and soil nitrogen during the initial phase of ecosystem development of the artificial watershed Hühnerwasser T2 - Geophysical Research Abstracts N2 - ln 2005, a 7-ha artificial watershed (Hühnerwasser) was built an a post mined Iandscape in Lusatia, Germany from sandy Substrates of Pleistocene origin, commonly used in reclamation. The watershed was developed to investigate the initial phase of soil and ecosystem development under natural conditions. At this early stage, nutrients in young sandy soils are limited and pioneer plants become key components of natural succession. Local abundant pioneering legumes Lotus corniculatus and Trifolium arvense and one pioneer grass species Calamagrostis epigeios were investigated 5 years after watershed construction. ln the present study, we investigated the influence of spatial root distribution of these species an soil nitrogen accumulation and on nutrient redistribution. Soil, including roots, was sampled from field monoliths covered with the aforementioned plant species. Root systems of both legumes were mainly restricted to the upper 20 cm of soil, whereas roots of C. epigeios also developed strongly at greater depths. A positive relationship was found, with higher plant densities associated with higher root densities, which were related with higher nodule densities for legumes and which were all associated with significantly higher soil nitrogen concentration relative to non-vegetated areas. This research provides rare information on the role root systems of pioneer legumes play in soil nitrogen input and development of nutrient patterns in the early stage of soil and ecosystem development during revegetation by natural succession. Y1 - 2019 UR - https://meetingorganizer.copernicus.org/EGU2019/EGU2019-5362.pdf VL - 21 ER - TY - GEN A1 - Boldt-Burisch, Katja A1 - Naeth, M. Anne A1 - Schneider, Bernd Uwe A1 - Hüttl, Reinhard F. T1 - Linkage between root systems of three pioneer plant species and soil nitrogen during early reclamation of a mine site in Lusatia, Germany T2 - Restoration Ecology N2 - In 2005, a 7-ha artificial watershed (Chicken Creek) was built on a post mined landscape in Lusatia, Germany from sandy substrates of Pleistocene origin, commonly used in reclamation. The watershed was developed to investigate the initial phase of soil and ecosystem development under natural conditions. At this early stage, mineral nitrogen in young sandy soils is primarily limited and nitrogen fixing legumes become key components of natural succession. Local abundant pioneering legumes Lotus corniculatus and Trifolium arvense and one pioneer grass species Calamagrostis epigeios were investigated 5 years after watershed construction. In this study, we investigated the influence of spatial root and nodule distribution of these species on soil nitrogen accumulation. Soil, including roots, was sampled from field monoliths covered with the aforementioned plant species. Root systems of both legumes were mainly restricted to the upper 20 cm of soil, whereas roots of C. epigeios also developed strongly at greater depths. A positive relationship was found, with higher plant densities associated with higher root densities which were associated with higher nodule densities for legumes and which were all associated with significantly higher soil nitrogen content relative to non-vegetated areas. This research provides rare information on the role root systems of pioneer legumes play in soil nitrogen input in the early stage of soil and ecosystem development during revegetation by natural succession. Y1 - 2015 U6 - https://doi.org/10.1111/rec.12190 SN - 1526-100X VL - 23 IS - 4 SP - 357 EP - 365 ER - TY - GEN A1 - Kwak, Jin-Hyeob A1 - Chang, Scott X. A1 - Naeth, M. Anne A1 - Schaaf, Wolfgang T1 - Coarse Woody Debris Increases Microbial Community Functional Diversity but not Enzyme Activities in Reclaimed Oil Sands Soils T2 - PLoS One N2 - Forest floor mineral soil mix (FMM) and peat mineral soil mix (PMM) are cover soils commonly used for upland reclamation post open-pit oil sands mining in northern Alberta, Canada. Coarse woody debris (CWD) can be used to regulate soil temperature and water content, to increase organic matter content, and to create microsites for the establishment of microorganisms and vegetation in upland reclamation. We studied the effects of CWD on soil microbial community level physiological profile (CLPP) and soil enzyme activities in FMM and PMM in a reclaimed landscape in the oil sands. This experiment was conducted with a 2 (FMM vs PMM) × 2 (near CWD vs away from CWD) factorial design with 6 replications. The study plots were established with Populus tremuloides (trembling aspen) CWD placed on each plot between November 2007 and February 2008. Soil samples were collected within 5 cm from CWD and more than 100 cm away from CWD in July, August and September 2013 and 2014. Microbial biomass was greater (p<0.05) in FMM than in PMM, in July, and August 2013 and July 2014, and greater (p<0.05) near CWD than away from CWD in FMM in July and August samplings. Soil microbial CLPP differed between FMM and PMM (p<0.01) according to a principal component analysis and CWD changed microbial CLPP in FMM (p<0.05) but not in PMM. Coarse woody debris increased microbial community functional diversity (average well color development in Biolog Ecoplates) in both cover soils (p<0.05) in August and September 2014. Carbon degrading soil enzyme activities were greater in FMM than in PMM (p<0.05) regardless of distance from CWD but were not affected by CWD. Greater microbial biomass and enzyme activities in FMM than in PMM will increase organic matter decomposition and nutrient cycling, improving plant growth. Enhanced microbial community functional diversity by CWD application in upland reclamation has implications for accelerating upland reclamation after oil sands mining. Y1 - 2015 U6 - https://doi.org/10.1371/journal.pone.0143857 SN - 1932-6203 VL - 10 SP - 0143857 ER - TY - GEN A1 - Kwak, Jin-Hyeob A1 - Chang, Scott X. A1 - Naeth, M. Anne A1 - Schaaf, Wolfgang T1 - Coarse woody debris effects on greenhouse gas emission rates depend on cover soil type in oil sands reclamation T2 - Applied Soil Ecology N2 - Peat mineral soil mix (PMM) and forest floor mineral soil mix (FMM) are cover soils commonly used for land reclamation, while coarse woody debris (CWD) can be added to create structural diversity and provide an additional source of organic matter. However, the effect of cover soil type and CWD on emission rates of greenhouse gases, such as carbon dioxide (CO₂) methane (CH₄) and nitrous oxide (N₂O) in reclaimed oil sands soils has not been studied. Soil respiration, CH₄ uptake and N₂O emission rates were studied in a factorial experiment consisting of 2 cover soils (FMM vs PMM) × 2 sampling distances from the CWD (near vs away from CWD). Greenhouse gas emission rates were measured in July, August, and September 2012 and 2013 using static chambers. Soil respiration rates were greater in FMM than in PMM regardless of the distance from CWD at each sampling time (p < 0.05). Rates ranged from 461 to 1148 and 293 to 677 mg CO2 m⁻²h⁻¹for FMM and PMM, respectively, in 2012, and from 355 to 1318 and 235 to 700 mg CO2 m⁻²h⁻¹, respectively, in 2013. The CWD increased soil respiration by 22–33% in FMM but not in PMM. Soil respiration rates were positively related to microbial biomass carbon (p = 0.004) and nitrogen (p < 0.001). Soil respiration rates decreased from July to September in 2012 and 2013, and were positively related to soil temperature (p < 0.01) but not with soil water content measured at 5 cm depth. Methane uptake rates were greater in FMM (0.026–0.037) than in PMM (0.015–0.028 mg CH₄⁻²h⁻¹. The CWD increased CH4 uptake rates only in July and August 2012 in FMM, and were negatively related to soil water content (p < 0.001) but not to soil temperature. Nitrous oxide emission rates (0.001–0.016 mg N₂O m⁻²h⁻¹) were not affected by either cover soil type or CWD. Global warming potential of CO₂, CH₄ and N₂O effluxes was greater in FMM than in PMM and near CWD than away from CWD, especially in FMM. Our study demonstrates that applying CWD for oil sands reclamation increases organic matter decomposition (increased CO₂ evolution), driven by the effect on microbial populations. Results from this study provide support to findings in earlier studies that CWD application benefits vegetation establishment through enhancing soil processes in reclaimed oil sands lands. Y1 - 2016 U6 - https://doi.org/10.1016/j.apsoil.2015.12.006 SN - 0929-1393 VL - 100 SP - 124 EP - 134 ER - TY - GEN A1 - Boldt-Burisch, Katja A1 - Naeth, M. Anne T1 - Early colonization of root associated fungal communities on reclamation substrates at a diamond mine in the Canadian Sub-Arctic T2 - Applied Soil Ecology N2 - Mining sites are harsh environments and establishment of root associated symbiotic fungi may be crucial for plant establishment and long term community development. Diamond mining in the Northwest Territories produces large amounts of processed kimberlite, and in some cases lake bed sediment and reclamation is needed for re-establishment of ecosystem function. This study investigated early fungal colonization with arbuscular mycorrhizae and dark septate endophytes on common reclamation substrates of different ages, relative to native tundra. Natural colonization of vegetation free sites with mycorrhizal spores on a trajectory associated with substrate age and type was very low. Fungal spore quantity and diversity was significantly accelerated by establishment of vegetation. Dark septate endophytes dominated native site Cyperaceae whereas reclamation site grasses were dominated by arbuscular mycorrhizae. Topsoil amendment was most effective for fungal colonization on reclamation substrates suggesting that a single application of topsoil can have a long term effect on the soil fungal community. Y1 - 2017 U6 - https://doi.org/10.1016/j.apsoil.2016.10.006 SN - 0929-1393 VL - 110 SP - 118 EP - 126 ER - TY - GEN A1 - Boldt-Burisch, Katja A1 - Naeth, M. Anne T1 - Mycorrhization affects root distribution of Lotus corniculatus and Calamagrostis epigeios in a nutrient poor heterogeneous soil in a rhizotron experiment T2 - Rhizosphere N2 - Plant growth can be highly dependent on symbiotic partners, such as arbuscular mycorrhizal fungi (AMF) that improve the plant's nutrient uptake. However, less is known about whether AMF can beneficially influence spatial root distribution, especially in soils with patchy nutrient concentrations. This study was conducted to evaluate root distribution and plant response of Lotus corniculatus and Calamagrostis epigeios in a heterogeneous nutrient poor soil under different mycorrhizal conditions. Heterogeneous soil conditions of a reclamation site in the Lusation mining district, consisting of a nutrient poor sandy soil with embedded large silt-clay fragments, were used for this rhizotron experiment. Treatments were non-mycorrhizal and mycorrhizal L. corniculatus and C. epigeios in sterile soil and in sterile soil with a fungal free soil solution and mycorrhizal plants in a non-sterile soil. Mycorrhizal fungi were exogenous (Funneliformis mosseae and Rhizophagus irregularis) in sterile soil and indigenous mycorrhizal fungi in non-sterile soil. Plants were grown in rhizotrons, with clay-silt fragments in a sandy soil matrix. Treatment response variables were root density, root and shoot biomass, shoot tissue nutrient concentrations, and percentage of roots in clay-silt fragments relative to total root biomass, mycorrhizal colonization and intraradical fungal alkaline phosphatase (ALP) activity. Exogenous mycorrhizal L. corniculatus plants had higher root mass density in clay-silt fragments than indigenous mycorrhizal and non-mycorrhizal plants while C. epigeios had only higher root mass density in clay-silt fragments relative to sandy soil only in exogenous mycorrhizal plants in sterile soil with soil solution. Nonmycorrhizal C. epigeios shoot biomass in sterile soil did not significantly differ from that of exogenous mycorrhizal treatments. The greatest shoot biomass for both plant species, was with the highest percentage of roots in clay-silt fragments relative to the total root system. Exogenous mycorrhizal plants of both species had highest shoot phosphorus concentrations in sterile soil with soil solution. However, intraradical fungal ALP activity was highest in indigenous mycorrhizal plants. The results indicate that mycorrhizal conditions can have a strong influence on spatial root development. The consequences are that conditions, which promote root growth, lead to more roots in nutrient rich clay-silt fragments and thus enhance plant exploitation of heterogeneously distributed soil nutrients. Y1 - 2017 U6 - https://doi.org/10.1016/j.rhisph.2017.06.003 SN - 2452-2198 VL - 4 SP - 36 EP - 47 ER - TY - GEN A1 - Boldt-Burisch, Katja A1 - Naeth, M. Anne A1 - Schneider, Uwe A1 - Schneider, Beate A1 - Hüttl, Reinhard F. T1 - Plant growth and arbuscular mycorrhizae development in oil sands processing by-products T2 - Science of the Total Environment N2 - Soil pollutants such as hydrocarbons can induce toxic effects in plants and associated arbuscular mycorrhizal fungi (AMF). This study was conducted to evaluate if the legume Lotus corniculatus and the grass Elymus trachycaulus and arbuscular mycorrhizal fungi could growin two oil sands processing by-products after bitumen extraction fromthe oil sands in northern Alberta, Canada. Substrate treatmentswere coarse tailings sand (CTS), a mix of dry mature fine tailings (MFT) with CTS (1:1) and Pleistocene sandy soil (hydrocarbon free); microbial treatments were without AMF, with AMF and AMF plus soil bacteria isolated from oil sands reclamation sites. Plant biomass, root morphology, leaf water content, shoot tissue phosphorus content and mycorrhizal colonization were evaluated. Both plant species had reduced growth in CTS and tailings mix relative to sandy soil. AMF frequency and intensity in roots of E. trachycaulus was not influenced by soil hydrocarbons; however, it decreased significantly over time in roots of L. corniculatus without bacteria in CTS. Mycorrhizal inoculation alone did not significantly improve plant growth in CTS and tailings mix; however, inoculation with mycorrhizae plus bacteria led to a significantly positive response of both plant species in CTS. Thus, combined inoculationwith selected mycorrhizae and bacteria led to synergistic effects. Such combinations may be used in future to improve plant growth in reclamation of CTS and tailings mix. Y1 - 2018 U6 - https://doi.org/10.1016/j.scitotenv.2017.11.188 SN - 1879-1026 SN - 0048-9697 VL - 621 SP - 30 EP - 39 ER - TY - GEN A1 - Boldt-Burisch, Katja A1 - Naeth, M. Anne T1 - Heterogeneous soil conditions influence fungal alkaline phosphatase activity in roots of Lotus corniculatus T2 - Applied Soil Ecology N2 - Extremes in soil water, temperature, pH and inorganic nutrient availability can highly influence mycorrhizal formation and nutrient acquisition. However, the influence of soil heterogeneity and seasonal soil changes on fungal alkaline phosphatase (ALP) activity in mycorrhizal roots is unknown. Whether the activity of intraradical fungal ALP is influenced by soil texture heterogeneity, soil depth, nutrient concentrations and root density during the growing season were addressed in this research. The root system of Lotus corniculatus grown on a reclamation site was analyzed for intraradical mycorrhizal ALP activity in 0–10, 10–20 and 20–30 cm depth increments of sandy soil and in clay–silt fragments (mostly localized in 10–20 cm) from May to September. Root length density and water soluble nutrient contents in sandy soil and clay–silt fragments were determined for each sample. Soil water content and soil temperature data of a nearby site were used for climate conditions on sampling dates. Fungal ALP activity in roots growing in clay–silt fragments was higher than in those growing in sandy soil, independent of sampling date. Fungal ALP activity in sandy soil increased with soil depth which is related to decreasing root densities and lower nutrient concentrations. However, nutrient dense clay–silt fragments significantly positively influenced local mycorrhizal ALP activity in L. corniculatus roots, suggesting that the ability of mycorrhizae to selectively take up phosphorus and other nutrients from those nutrient hot spots is enhanced and could play a significant role in successful establishment of pioneer plant species on infertile reclamation sites. Y1 - 2017 U6 - https://doi.org/10.1016/j.apsoil.2017.03.024 SN - 0929-1393 VL - 116 SP - 55 EP - 63 ER - TY - GEN A1 - Stover, Holly J. A1 - Naeth, M. Anne A1 - Boldt-Burisch, Katja T1 - Soil disturbance changes arbuscular mycorrhizal fungi richness and composition in a fescue grassland in Alberta Canada T2 - Applied Soil Ecology N2 - Native grasslands are endangered by non-native plant invasion worldwide, including foothills fescue grasslands in North America. Large populations of non-native plant species have established in these disturbed fescue grasslands, forming dense monocultures and spreading into undisturbed areas. Soil disturbance and plant invasion can alter the arbuscular mycorrhizal fungi (AMF) community, an important symbiotic partner of most land plants, which could negatively affect native plant reestablishment. The objective of this study was to assess whether AMF communities on a fescue grassland shifted in response to disturbances by landfill storage and gravel quarrying and with invasion of non-native plant species relative to undisturbed grassland. Soil and root-AMF samples were procured from disturbed and undisturbed areas at three sites. Plant canopy cover and species richness were assessed. Soils were analyzed for pH; electrical conductivity; total nitrogen, carbon and phosphorus; and available nutrients. For relative AMF taxa abundance assessment, NS31 and AMF specific primer AML2 were used to amplify a central fragment of the V3 and V4 region of the 18S rRNA gene. AMF were characterized using 454 pyrosequencing and multiplexed barcoded samples amplified from genomic DNA isolated from roots. There were 92 AMF, including 15 potentially novel taxa detected. AMF communities in disturbed and undisturbed sampling locations were distinct except for one site, and indicator AMF virtual taxa (VT) for undisturbed grassland and disturbed sites were identified. AMF richness was higher in undisturbed (72 VT) than disturbed (64 VT) sites and AMF richness was positively correlated with plant species richness, diversity and native plant cover, and negatively correlated with non-native plant cover. There were 43 AMF VT on undisturbed and disturbed sites, 62% with higher relative abundance on disturbed sites. Site disturbance shifted AMF communities relative to undisturbed native fescue grassland; thus restoration success with native plants might be highly dependent on reintroducing native AMF. Y1 - 2018 U6 - https://doi.org/10.1016/j.apsoil.2018.07.008 SN - 0929-1393 VL - Vol. 131 SP - 29 EP - 37 ER - TY - GEN A1 - Boldt-Burisch, Katja A1 - Naeth, M. Anne T1 - Phosphogypsum significantly alters root growth and fungal colonization of smooth brome and sheep fescue on a reclamation site T2 - Rhizosphere N2 - Phosphogypsum (PG) is a by-product of phosphate fertilizer production. This study is the first to investigate Bromis inermis and Festuca ovina root morphology and colonization with arbuscular mycorrhizal fungi and dark septate endophytes (DSE) in pure PG and PG with a soil cap (soil). Root length density was significantly lower for plants on PG than on soil and both plant species were colonized with AMF and DSE, with lower frequency and intensity in PG. Phosphorus (20 times), calcium (35 times) and sulphur (50 times) were significantly higher in PG than in soil; resulting higher root tissue concentrations were significantly negatively correlated with mycorrhizal colonization for Festuca but not Bromus. Higher fungal colonization of Bromus, especially with DSE known to be prevalent in extreme environments, may be responsible for its better performance on PG relative to Festuca. Y1 - 2019 U6 - https://doi.org/10.1016/j.rhisph.2018.12.003 SN - 2452-2198 VL - 9 SP - 106 EP - 109 ER - TY - CHAP A1 - Boldt-Burisch, Katja A1 - Naeth, M. Anne A1 - Schneider, Bernd Uwe A1 - Hüttl, Reinhard F. T1 - Root growth, mycorrhization and physiological effects of plants growing on oil tailing sands T2 - European Geosciences Union, General Assembly 2015, Vienna, Austria, 13 April - 17 May 2015 Y1 - 2015 UR - http://meetingorganizer.copernicus.org/EGU2015/EGU2015-8991-1.pdf N1 - EGU2015-8991-1 PB - European Geophysical Society CY - Katlenburg-Lindau ER -